杭州浙大迪迅生物基因工程有限公司JACI：组份分析是基于过敏原提取物的致敏性分析的补充
发布日期：2019-01-17
原标题：分子学过敏原特异性IgE检测是基于过敏原提取物的致敏性分析的补充
延伸阅读
JACI：
http://dx.doi.org/10.1016/j.jaip.2015.09.013
Molecular allergen- based component-resolved diagnostic IgE antibody tests have emerged in the form of singleplex assays and multiplex arrays. They use both native and recombinant allergen molecules, sometimes in combination with each other, to supplement allergen extract-based IgE antibody analyses. The total number of available allergenic molecules has reached a diagnostically useful level; however, more molecules are needed to cover all the clinically important allergen specificities. Thus, for the foreseeable future, molecular allergen-specific IgE analyses will remain a supplement for initial allergen extract-based IgE antibody analyses in the diagnostic workup of the allergic patient. As a spin-off, it will enable manufacturers to improve the quality of extracts for in vitro testing. The 2 most exciting diagnostic developments linked to component-resolved diagnostic tests are the possibility to increase diagnostic sensitivity by the inclusion of allergens that are underrepresented in the current extracts and in vitro assays and to increase the diagnostic specificity by taking the information on allergen cross-reactivity into account. Particularly the latter application is still under development. This requires additional studies on the clinical relevance of serological cross-reactivity. 2015 American Academy of Allergy, Asthma & Immunology ( J Allergy Clin Immunol Pract 2015;3:863-9)
All Authors:
Rob C.AalbersePhDabJoost A.AalberseMD
——浙大迪迅 译
基于过敏原分子组份分析的诊断性的IgE抗体检测已经以单一和多重阵列检测的形式出现。他们同时使用天然和重组的过敏原分子(有时相互结合)来补充基于提取物的过敏原IgE抗体分析。可获得的致敏分子总数已达到有助于诊断的水平;然而，需要更多的分子来涵盖所有临床上重要过敏原的特异性。因此，在可预见的未来，分子过敏原特异性IgE分析仍将是过敏原提取物IgE抗体分析在过敏诊断中的补充。因此，它将促使制造商能够提高用于体外检测的提取物的质量。与组份诊断检测相关的两个最喜人的诊断进展是，有可能通过包含当前提取物和体外分析中未充分表达的过敏原来提高诊断灵敏度，并通过考虑过敏原交叉反应性信息来提高诊断特异性。特别是后者的应用仍在开发中。这就需要进一步研究血清学交叉反应的临床相关性。2015美国过敏、哮喘与免疫学学会(J Allergy Clin Immunol Pract 2015;3:863-9)
延伸阅读
JACI：
[IF:13.258]
Molecular Allergen-Specific IgE Assays as a Complement to Allergen Extract-Based Sensitization Assessmenthttp://dx.doi.org/10.1016/j.jaip.2015.09.013
Molecular allergen- based component-resolved diagnostic IgE antibody tests have emerged in the form of singleplex assays and multiplex arrays. They use both native and recombinant allergen molecules, sometimes in combination with each other, to supplement allergen extract-based IgE antibody analyses. The total number of available allergenic molecules has reached a diagnostically useful level; however, more molecules are needed to cover all the clinically important allergen specificities. Thus, for the foreseeable future, molecular allergen-specific IgE analyses will remain a supplement for initial allergen extract-based IgE antibody analyses in the diagnostic workup of the allergic patient. As a spin-off, it will enable manufacturers to improve the quality of extracts for in vitro testing. The 2 most exciting diagnostic developments linked to component-resolved diagnostic tests are the possibility to increase diagnostic sensitivity by the inclusion of allergens that are underrepresented in the current extracts and in vitro assays and to increase the diagnostic specificity by taking the information on allergen cross-reactivity into account. Particularly the latter application is still under development. This requires additional studies on the clinical relevance of serological cross-reactivity. 2015 American Academy of Allergy, Asthma & Immunology ( J Allergy Clin Immunol Pract 2015;3:863-9)
All Authors:
Rob C.AalbersePhDabJoost A.AalberseMD
2018-12-28 Article
创建过敏性疾病的科研、科普知识交流平台，为过敏患者提供专业诊断、治疗、预防的共享平台。
