杭州浙大迪迅生物基因工程有限公司Clinical & Experimental Allergy：特应性皮炎患者与呼吸道过敏患者对屋尘螨过敏原组份的敏感性特征分析
发布日期：2019-01-02
原标题：特应性皮炎患者对各种屋尘螨过敏原中次要组分的敏感性高于呼吸道过敏性疾病患者
延伸阅读
Clinical & Experimental Allergy：
https://doi.org/10.1111/cea.13164
Background
Various allergenic proteins are produced by house dust mites (HDM). However, the allergenicity and clinical implications of these allergens are unknown.
Objective
The purpose of this study was to identify allergens in Dermatophagoides farinae and elucidate the sensitization profiles to these in Korean patients suffering from respiratory (allergic rhinitis and/or asthma) and atopic dermatitis symptoms.
Methods
IgE reactivities in sera from 160 HDM allergy patients were analysed by one‐ and two‐dimensional gel electrophoresis and immunoblotting. IgE‐reactive components were identified by liquid chromatography‐coupled electrospray ionization‐tandem mass spectrometry. Nine recombinant mite allergens (Der f 1, Der f 2, Der f 10, Der f 11, Der f 13, Der f 14, Der f 30, Der f 32 and Der f Alt a 10) were produced, and the IgE reactivity in sera to each was determined by ELISAs.
Results
Der f 1 and Der f 2 were recognized by IgE in serum samples from 88.1% and 78.1% of all patients, respectively. Patients with respiratory allergies were mainly sensitized to these major allergens, whereas patients with atopic dermatitis symptoms showed polysensitization to major and minor allergen components (including Der f 11, Der f 13, Der f 14, Der f 32 and Der f Alt a 10).
Conclusions
Patients with respiratory allergic disease sensitize to major allergen components of HDM. Those with atopic dermatitis were sensitized to a broader range of minor allergen components of HDM (Der f 11, Der f 13, Der f 14, Der f 32 and Der f Alt a 10).
All Authors
K. H. Park J. Lee J.‐Y. Lee S. C. LeeD. W. SimJ. U. ShinC. O. ParkJ.‐H. LeeK. H. LeeK. Y. Jeong
——来自浙大迪迅
①室内尘螨(HDM)可产生多种致敏蛋白。然而，这些过敏原的致敏性和临床意义尚不清楚。②本研究的目的是确定粉尘螨过敏原，并阐明韩国患有呼吸道(变应性鼻炎和/或哮喘)和特应性皮炎症状的患者对这些过敏原的敏感性情况。③对160例HDM过敏患者血清IgE反应性进行单、二维凝胶电泳和免疫印迹分析。IgE‐活性组分采用液相色谱-耦合电喷雾电离-串联质谱法测定。产生9种重组螨变应原(Der f1、Der f2、Der f10、Der f11、Der f13、Der f14、Der f30、Der f32、Der f Alt a 10)， ELISAs测定血清中IgE对上述组份的反应活性。④ Der f1和Der f2分别被88.1%和78.1%患者的血清IgE识别。呼吸道过敏患者主要对这两种主要过敏原敏感，特应性皮炎患者对主要和次要过敏原成分(包括Der f11、Der f13、Der f14、Der f32和Der falt a10)表现出多重致敏性。⑤呼吸道过敏性疾病患者对HDM的主要过敏原组份敏感。特应性皮炎患者对HDM中更广泛的次要过敏原组份(Der f11、Der f13、Der f14、Der f32和Der falt a10)敏感。
延伸阅读
Clinical & Experimental Allergy：
[IF:6.05]
Sensitization to various minor house dust mite allergens is greater in patients with atopic dermatitis than in those with respiratory allergic diseasehttps://doi.org/10.1111/cea.13164
Background
Various allergenic proteins are produced by house dust mites (HDM). However, the allergenicity and clinical implications of these allergens are unknown.
Objective
The purpose of this study was to identify allergens in Dermatophagoides farinae and elucidate the sensitization profiles to these in Korean patients suffering from respiratory (allergic rhinitis and/or asthma) and atopic dermatitis symptoms.
Methods
IgE reactivities in sera from 160 HDM allergy patients were analysed by one‐ and two‐dimensional gel electrophoresis and immunoblotting. IgE‐reactive components were identified by liquid chromatography‐coupled electrospray ionization‐tandem mass spectrometry. Nine recombinant mite allergens (Der f 1, Der f 2, Der f 10, Der f 11, Der f 13, Der f 14, Der f 30, Der f 32 and Der f Alt a 10) were produced, and the IgE reactivity in sera to each was determined by ELISAs.
Results
Der f 1 and Der f 2 were recognized by IgE in serum samples from 88.1% and 78.1% of all patients, respectively. Patients with respiratory allergies were mainly sensitized to these major allergens, whereas patients with atopic dermatitis symptoms showed polysensitization to major and minor allergen components (including Der f 11, Der f 13, Der f 14, Der f 32 and Der f Alt a 10).
Conclusions
Patients with respiratory allergic disease sensitize to major allergen components of HDM. Those with atopic dermatitis were sensitized to a broader range of minor allergen components of HDM (Der f 11, Der f 13, Der f 14, Der f 32 and Der f Alt a 10).
All Authors
K. H. Park J. Lee J.‐Y. Lee S. C. LeeD. W. SimJ. U. ShinC. O. ParkJ.‐H. LeeK. H. LeeK. Y. Jeong
2018-12-27 Article
创建过敏性疾病的科研、科普知识交流平台，为过敏患者提供专业诊断、治疗、预防的共享平台。
