杭州浙大迪迅生物基因工程有限公司Clin Exp Allergy: 2S蛋白Ara h7.0201与2S蛋白Ara h2、6嗜碱性细胞脱粒能力比较
发布日期：2018-11-13
原标题：2S蛋白Ara h7.0201与其他蛋白Ara h7亚型相比具有独特的表位，在嗜碱性细胞脱粒能力上与2S蛋白Ara h2、6具有可比性
延伸阅读
Clinical & Experimental Allergy
DOI: 10.1111/cea.13134
Abstract:
Background: Screening for specific IgE against 2S albumin proteins Ara h 2 and 6 has good positive predictive value in diagnosing peanut allergy. From the third 2S member Ara h 7, 3 isoforms have been identified. Their allergenicity has not been elucidated.
Methods: Sensitization of 15 DBPCFC-confirmed peanut-allergic patients to recombinant Ara h 2.0201, Ara h 6.01 and isoforms of recombinant Ara h 7 was determined by IgE immunoblotting strips. A basophil activation test (BAT) was performed in 9 patients to determine IgE-cross-linking capacities of the allergens. Sensitivity to the allergens was tested in 5 patients who were sensitized to at least 1 Ara h 7 isoform, by a concentration range in the BAT. 3D prediction models and sequence alignments were used to visualize differences between isoforms and to predict allergenic epitope regions.
Results: Sensitization to Ara h 7.0201 was most frequent (80%) and showed to be equally potent as Ara h 2.0201 and 6.01 in inducing basophil degranulation. Sensitization to Ara h 7.0201 together with Ara h 2.0201 and/or 6.01 was observed, indicating the presence of unique epitopes compared to the other 2 isoforms.Differences between the 3 Ara h 7 isoforms were observed in C-terminal cysteine residues, pepsin and trypsin cleavage sites and 3 single amino acid substitutions.
Conclusions: The majority of peanut-allergic patients are sensitized to isoform Ara h 7.0201, which is functionally as active as Ara h 2.0201 and 6.01. Unique epitopes are most likely located in the C-terminus or an allergenic loop region which is a known allergenic epitope region for Ara h 2.0201 and 6.01. Due to its unique epitopes and allergenicity, it is an interesting candidate to improve the diagnostic accuracy for peanut allergy.
First Author:
S. M. Hayen
Correspondence:
Simone Hayen, Department of Dermatology/Allergology, UMC Utrecht, Utrecht, The Netherlands
All Authors:
S. M. Hayen, A. M. Ehlers, C. F. den Hartog Jager, J. Garssen, E. F. Knol, A. C. Knulst, W. Suer, L. E. M. Willemsen, H. G. Otten
——来自浙大迪迅
① 2S白蛋白对花生过敏有良好的预测价值，第三个2s蛋白Ara h7鉴定出3个亚型，其变应原特性还未被阐明；② 用免疫印迹法检测15例DBPCFC确诊的花生过敏患者对重组Ara h 2.0201、Ara h 6.01及重组Ara h 7亚型的敏感性；③ 9例患者中进行嗜碱性粒细胞激活试验（BAT），以确定过敏原的IgE耦合能力；④ 病人对Ara h 7.0201的敏感性最高（80%），并且在诱导嗜碱性粒细胞脱粒方面Ara h 7.0201与Ara h 2.0201和6.01一样活跃；⑤Ara h7.0201与其它2种异构体相比，存在独特的表位。
延伸阅读
Clinical & Experimental Allergy
[IF:5.158]
2S protein Ara h 7.0201 has unique epitopes compared to other Ara h 7 isoforms and is comparable to 2S proteins Ara h 2 and 6 in basophil degranulation capacityDOI: 10.1111/cea.13134
Abstract:
Background: Screening for specific IgE against 2S albumin proteins Ara h 2 and 6 has good positive predictive value in diagnosing peanut allergy. From the third 2S member Ara h 7, 3 isoforms have been identified. Their allergenicity has not been elucidated.
Methods: Sensitization of 15 DBPCFC-confirmed peanut-allergic patients to recombinant Ara h 2.0201, Ara h 6.01 and isoforms of recombinant Ara h 7 was determined by IgE immunoblotting strips. A basophil activation test (BAT) was performed in 9 patients to determine IgE-cross-linking capacities of the allergens. Sensitivity to the allergens was tested in 5 patients who were sensitized to at least 1 Ara h 7 isoform, by a concentration range in the BAT. 3D prediction models and sequence alignments were used to visualize differences between isoforms and to predict allergenic epitope regions.
Results: Sensitization to Ara h 7.0201 was most frequent (80%) and showed to be equally potent as Ara h 2.0201 and 6.01 in inducing basophil degranulation. Sensitization to Ara h 7.0201 together with Ara h 2.0201 and/or 6.01 was observed, indicating the presence of unique epitopes compared to the other 2 isoforms.Differences between the 3 Ara h 7 isoforms were observed in C-terminal cysteine residues, pepsin and trypsin cleavage sites and 3 single amino acid substitutions.
Conclusions: The majority of peanut-allergic patients are sensitized to isoform Ara h 7.0201, which is functionally as active as Ara h 2.0201 and 6.01. Unique epitopes are most likely located in the C-terminus or an allergenic loop region which is a known allergenic epitope region for Ara h 2.0201 and 6.01. Due to its unique epitopes and allergenicity, it is an interesting candidate to improve the diagnostic accuracy for peanut allergy.
First Author:
S. M. Hayen
Correspondence:
Simone Hayen, Department of Dermatology/Allergology, UMC Utrecht, Utrecht, The Netherlands
All Authors:
S. M. Hayen, A. M. Ehlers, C. F. den Hartog Jager, J. Garssen, E. F. Knol, A. C. Knulst, W. Suer, L. E. M. Willemsen, H. G. Otten
2018-10-25 Article
创建过敏性疾病的科研、科普知识交流平台，为过敏患者提供专业诊断、治疗、预防的共享平台。
