杭州浙大迪迅生物基因工程有限公司JACI：蜜蜂毒液过敏原组份诊断的新发现
发布日期：2018-12-18
原标题：蜜蜂毒液过敏病人中应用过敏原组份诊断发现新的主要过敏原
——来自浙大迪迅
①重组膜翅目毒液过敏原的IgE检测被认为可以提高膜翅目毒液过敏的诊断正确性。然而，对蜜蜂毒液过敏患者唯一可用的重组蜂毒(HBV)过敏原rApi m1的致敏频率是有限的，这表明可能存在另外的蜜蜂毒液过敏原。②我们对蜜蜂毒液过敏病人进行一系列蜜蜂毒液过敏原（组份）的敏感性分析。蜜蜂毒液过敏（HBV）的诊断(n = 144)是基于病史、皮肤试验结果和对HBV的过敏原特异性IgE水平。采用ImmunoCAP分析IgE对6种无交叉反应碳水化合物(CCD)的HBV过敏原的反应性。④.IgE对rApi m1、rApi m2、rApi m3、nApi m4、rApi m5、rApi m10的反应频率分别为72.2%、47.9%、50.0%、22.9%、58.3%、61.8%。94.4%的患者对至少1种HBV过敏原呈阳性反应。在68.0%的患者中检测到IgE对Api m3、Api m10或两者都有反应，并在5%的患者中是唯一的HBV过敏原特异性IgE。治疗性HBV对IgE结合的抑制有限，在获得免疫治疗的患者中对Api m3和Api m 10特异性IgG4的诱导有限，这些都支持了最近关于这些过敏原在治疗性HBV制剂中表达不足的报道。⑤与单独使用rApi m1相比，对一组无CCD（碳水化合物交叉反应决定簇）的HBV过敏原（组份）的分析提高了诊断敏感性，识别了其它主要过敏原，并揭示了在治疗性HBV制剂中已报道的缺失或未充分表达的过敏原（组份）的敏感性。
延伸阅读
JACI:
[IF:13.1]
Component resolution reveals additional major allergens in patients with honeybee venom allergy
https://doi.org/10.1016/j.jaci.2013.10.060
Abstract:
Background
Detection of IgE to recombinant Hymenoptera venom allergens has been suggested to improve the diagnostic precision in Hymenoptera venom allergy. However, the frequency of sensitization to the only available recombinant honeybee venom (HBV) allergen, rApi m 1, in patients with HBV allergy is limited, suggesting that additional HBV allergens might be of relevance.
Objective
We performed an analysis of sensitization profiles of patients with HBV allergy to a panel of HBV allergens.
Methods
Diagnosis of HBV allergy (n = 144) was based on history, skin test results, and allergen-specific IgE levels to HBV. IgE reactivity to 6 HBV allergens devoid of cross-reactive carbohydrate determinants (CCD) was analyzed by ImmunoCAP.
Results
IgE reactivity to rApi m 1, rApi m 2, rApi m 3, nApi m 4, rApi m 5, and rApi m 10 was detected in 72.2%, 47.9%, 50.0%, 22.9%, 58.3%, and 61.8% of the patients with HBV allergy, respectively. Positive results to at least 1 HBV allergen were detected in 94.4%. IgE reactivity to Api m 3, Api m 10, or both was detected in 68.0% and represented the only HBV allergen–specific IgE in 5% of the patients. Limited inhibition of IgE binding by therapeutic HBV and limited induction of Api m 3– and Api m 10–specific IgG4 in patients obtaining immunotherapy supports recent reports on the underrepresentation of these al lergens in therapeutic HBV preparations
Conclusion
Analysis of a panel of CCD-free HBV allergens improved diagnostic sensitivity compared with use of rApi m 1 alone, identified additional major allergens, and revealed sensitizations to allergens that have been reported to be absent or underrepresented in therapeutic HBV preparations.
All Author:
JulianKöhlerMSa∗SimonBlankPhDb∗SabineMüllerMDaFrankBantleonDiplBiolbMarcelFrickMSaJohannesHuss-MarpMDacJonasLidholmPhDdEdzardSpillnerPhDb‡ThiloJakobMDa‡
2018-12-10 Article
创建过敏性疾病的科研、科普知识交流平台，为过敏患者提供专业诊断、治疗、预防的共享平台。
